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Complete Genome Sequence of a 2019 Novel Coronavirus(SARS-CoV-2) Strain Causing a COVID-19 Case in Morocco

Sanaâ Lemriss, Amal Souiri, Narjis Amar, Nabil Lemzaoui, Omar Mestoui, Mohamed Labioui, Nabil Ouaariba, Ayoub Jibjibe, Mahmoud Yartaoui, Mohamed Chahmi, Marouane El Rhouila, Samiha Sellak, Nadia Kandoussi, Saâd El Kabbaj. Complete Genome Sequence of a 2019 Novel Coronavirus(SARS-CoV-2) Strain Causing a COVID-19 Case in Morocco

ABSTRACT: Here, we report a complete genome sequence obtained for a novel severeacute respiratory syndrome coronavirus 2 (SARS-CoV-2) strain isolated from a nasopha-ryngeal swab specimen of a Moroccan patient with coronavirus disease 2019 (COVID-19)

Microbiology Resource Announcements-2020-Lemriss-e00633-20.full

Etude épidémiologique de l’infection à Hélicobacter pylori dans la région de Rabat

K Laarej, R Alami, L Bentaleb, M Jbilou, S El Kabbaj, M Elmaouardi and A Bouklouze

Infection with H. pylori is universal, likely to affect all populations, but especially encountered when socio-economic conditions are unfavorable. The prevalence of this infection is 70 to 90% in developing countries, while it represents only 20 to 30% in industrialized countries. To assess the prevalence of H. pylori infection in the Rabat-Sale-Zamour-Zaer region, we present a retrospective cohort made up of 3923 patients with gastric symptoms admitted to the LRAM from early 2016 to the end of 2018 for a diagnosis of H. pylori infection by the 13C-labeled urea breath test. The overall prevalence rate of H. pylori infection in this study is 35.89% distributed equally between the two sexes, but partially high among the youngest age group between 14 and 39 years (41.24%)

Epidemiological Study of Helicobacter Pylori Infection in a Population in the Rabat-Sale-Zamour-Zaer Region

Recherche de résidus de pesticides dans les fraises au Maroc

Mohammed Jbilou, Khalid Laarej, Rachid Alami

Strawberries, fruits of the family Rosaceae (Fragaria vulgaris L), grown in Morocco in the regions of Souss, Gharb and Loukkos, are widely consumed because of their high vitamin content (C, B1, P and PP) and in mineral salts. This crop is very sensitive to parasites which requires treatments with pesticides to preserve the crop. Our job is to develop a new analytical method based on the extraction of pesticide residues from strawberries samples and their GC-MS assay. The sample preparation consists of crushing the fruit and weighing 10 g for extraction by theQuECHERS method and 3 μl of the extract are injected into the GC-MS.The recovery percentage of this method is 87.5%, the detection limitsare 10 PPB and the linearity repetition coefficient is greater than 0.99.17 samples taken on the market were analyzed by this.

Development of A New Essay Method of Pesticides in Strawberries by GC-MS and Determination of their Residues in A Few Samples

Dosage des résidus de pesticides par GC-MS dans des échantillons de la menthe commercialises au Maroc

Mohammed Jbilou1, Khalid Laarej1, Rachid Alami1*, Abdelaziz Bouklouze2, Yahia Cherrah2, Adil El Yadini3 and My Elabbes Faouzi4

The sale of fresh mint in Morocco is not strictly controlled as for that which is intended for export to the European Union, which increases the risks of the contamination of mint tea consumers and constitutes a problem of public health. The method of dosing pesticides in fresh mint by GC-MS that we have developed and validated allowed us to high light contamination by pesticide residues (carbofuran, bifenthrin, chlopyriphos methyl and Fenpropathrin) in samples of mint fresh from sampled sale and temara, this contamination exceeds the MRLs. Regular control of the sale of fresh mint should be practiced to prevent repetitive contamination of local consumers by pesticide residues.

1Research Laboratory and Medical Analysis of the Royal Gendarmerie, Rabat, Morocco
2Research Team – Pharmaceutical Pharmacology and Toxicology Laboratory, Faculty of Medicine and Pharmacy, Mohammed V University in Rabat, Morocco
3Laboratory of Spectroscopy, Molecular Modelisation, Material and Environment (LS3ME), Faculty of Sciences, University Med V-Agdal, Avenue Ibn Battouta, BP 1014, Agdal, Rabat, Morocco
4Pharmacocinetic Team Laboratory of Pharmacology and Toxicology, Faculty of Medicine and Pharmacy, Mohammed V University in Rabat, Morocco

Pour consulter l’article dans son intégralité, Merci de contacter directement le Pr. Mohammed Jbilou à cette adresse: mjbilou@lram-fgr.ma

 

DEVELOPMENT AND VALIDATION OF AN ASSAY OF PHOSMET RESIDUES IN HUMAN BLOOD BY GC/MS

Rachid Alami, Mohammed Jbilou, Yahia Cherrah, Abdelaziz Bouklouze, Adil El Yadini and My Elabbes Faouzi. Development and validation of an assay of Phosmet residues in human blood by GC/MS Der Pharma Chemica, 2016, 8(3):26-33

Abstract:

An assay of phosmet residues in human blood by gas chromatography coupled to mass spectrometry (GC/MS) Clarus® 600/560 DMS PerkinElmer® was developed and validated in human blood samples loaded by residues of this insecticide. The extraction of phosmet has been carried out in solid phase through C18 SPE cartridges. The proposed method of phosmet residue analysis by GC/MS uses a capillary type Supelco®, the oven temperature was programmed from 75°C to 320°C. The Helium was used as carrier gas with a flow rate of 0.8 ml / min. The Phosalone was used as internal standard (IS). The retention time of the analyte and EI are 14.34 and 14.67 ± 0.02 min, respectively. The calibration curve is linear in the concentration range between 10 and 100ppb with R2 = 0.998. The method was validated according to ICH guidelines Q2 (R1) in terms of the criteria of accuracy, precision, linearity and specificity. The validated method is selective and has a quantification limit of 10 ppb. It can be used routinely for the determination of phosmet residues in real samples of human blood.

DEVELOPMENT AND VALIDATION OF A NEW ASSAY OF PESTICIDES RESIDUES IN HUMAN BLOOD BY GC/MS

Rachid Alami, Mohammed Jbilou, Yahia Cherrah, Abdelaziz Bouklouze, Adil El Yadini, & My Elabbes Faouzi. (2016). Development and validation of a new assay of pesticides residues in human blood by GC/MS. International Journal of Medical Research and Pharmaceutical Sciences: 3:4, 12-22. http://doi.org/10.5281/zenodo.50335

 Abstract:

A new method for pesticide residues analysis in human blood by gas chromatography coupled with mass spectroscopy (GC / MS) was developed and validated on human plasma samples loaded by pesticide residues (demethoate, endosulfan, befinthrin, phosmet and deltamethrin). The extraction of these pesticides was conducted in solid phase through C18 SPE cartridges.The method of analysis of pesticide residues by GC / MS proposed, using a capillary column supelco® type oven temperature was programmed from 75 ° C to 320 ° C. Helium was used as carrier gas with a flow rate of 0.8ml / min. Phosalone (100 ppb) was used as internal standard (EI). Calibrations curves pesticides are linear in a concentration range between 10 and 100 ppb with R2˃ 0.994 for all the pesticides. The method was validated according to the guidelines of the NF V03-110, in May 2010 in terms of the criteria of accuracy, fidelity, linearity and specificity. The validated method is selective and has a quantification limit of 10 ppb. It can be used routinely for the determination of pesticide residues in samples of human blood.

SCREENING OF PESTICIDE RESIDUES IN BLOOD BY GC-MS IN PATIENTS ADMITTED TO THE DEPARTMENT OF ENDOCRINOLOGY IBN SINA CHU RABAT

Rachid Alami, Mohammed Jbilou, Yahia Cherrah, Khalid Laarej, Adil El Yadini, Hind Iraqi, Abdelmjid Chraibi & My Elabbes Faouzi ( 2016) . Screening of pesticide residues in blood by GC-MS in patients admitted to the department of endocrinology ibn sina chu rabat. Der Pharmacia Lettre, 2016, 8 (19):78-85.

Abstract:

Repeated exposure or consumption of food contaminated with pesticide residues can cause various disorders for the human body, especially the immune and hormonal disturbances and the onset of certain cancers. The multirésiduel assay method of pesticides in blood by (GC-MS) Clarus® 600/560 DMS PerkinElmer® we developed allowed us to conduct a screening of the majority of pesticide residues used in Moroccan agriculture. The calibration range contains a mixture of the most used pesticides. The extraction was performed in a solid medium by CSPE cartridges. This method uses a capillary column of 30 meters type Supelco®, the oven temperature was programmed from 90 ° C to 290 ° C with a gradient of 10 ° C / min. Helium was used as carrier gas with a flow rate of 0.8ml / min. Phosalone was used as internal standard (IS). The calibration curves of different pesticides are linear with concentrations ranging between 10 and 70 ppb with satisfactory correlation coefficients (R2> 0.99) and CV <20% for all results. In these analytical conditions we analyzed blood samples of Endocrinology of the University Hospital of Rabat. The results showed that some patients are contaminated with pesticide residue; dimethoate and bifenthrin. The hypothesis of the involvement of these pesticides in the development of hormonal disorders in patients is very likely. This method may be expanded and supplemented to prove this causal link.

Keywords: Pesticides, GC-MS, assay, Patients.

Analysis of the Moroccan GBEA in comparison to the ISO 15189

Nadia Charrat. Analysis of the Moroccan GBEA in comparison to the ISO 15189. Conference : 13th Arab Congress of Clinical Biology and Moroccan & 12th Congress of Clinical Chemistry. Arabmedhealth Laboratory Medicine. Palais des Congrès Marrakech. 5 May 2012.