DEVELOPMENT AND VALIDATION OF A NEW ASSAY OF PESTICIDES RESIDUES IN HUMAN BLOOD BY GC/MS

A new method for pesticide residues analysis in human blood by gas chromatography coupled with mass spectroscopy (GC / MS) was developed and validated on human plasma samples loaded by pesticide residues (demethoate, endosulfan, befinthrin, phosmet and deltamethrin). The extraction of these pesticides was conducted in solid phase through C18 SPE cartridges.The method of analysis of pesticide residues by GC / MS proposed, using a capillary column supelco® type oven temperature was programmed from 75 ° C to 320 ° C. Helium was used as carrier gas with a flow rate of 0.8ml / min. Phosalone (100 ppb) was used as internal standard (EI). Calibrations curves pesticides are linear in a concentration range between 10 and 100 ppb with R2˃ 0.994 for all the pesticides. The method was validated according to the guidelines of the NF V03-110, in May 2010 in terms of the criteria of accuracy, fidelity, linearity and specificity. The validated method is selective and has a quantification limit of 10 ppb. It can be used routinely for the determination of pesticide residues in samples of human blood.